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SARAVANA KUMAR
Dr. SARAVANA KUMAR KAILASAM MANI

Affiliation :
Purdue University, West Lafayette,United States

Biography :

Post-Doctoral Research Associate, Purdue University, West Lafayette, IN, USA August 2017- present. Investigating the mechanism of sorafenib resistance in chronic Hepatitis B Virus induced liver cancer Graduate Research Assistant, Purdue University, West Lafayette, IN, USA August 2011 - May 2017. Identified a novel mechanism of cancer stem cell development in Hepatitis B Virus induced liver cancer. Validated the mechanism in human tissues & mice models, proposed new therapeutic targets to treat liver cancer. Collaborated with multiple labs and core facilities (bioinformatics, sequencing, imaging and flow cytometry) resulting in 5 high impact factor publications. Supervised and trained co-workers in cell and molecular biology techniques as well as in operating instruments like Flow Cytometers (BD LSR Fortessa), QPCR (LightCycler 96, ViiA7), Confocal & Fluorescence Microscopes (Olympus Fluoview, Nikon Eclipse 90i), Luminometers (Glomax), Seahorse Bioanalyzer.

Research Interest :

Cell Biology: Tissue culture (Immortalized cell lines, primary cell culture, human embryonic stem cells), Flow Cytometry (Single & Multi Color), Fluorescence & Confocal microscopy, Reporter Assays, Cell proliferation and Metabolic Assays

Proteomics: Protein purification & quantitation, Western blot, Chromatin Immunoprecipitation, ELISA

Genomics: Preparation and Quality Control of RNA Seq and ATAC Seq libraries, DNA/ RNA/microRNA purification & quantitation, Gel electrophoresis, Real Time PCR, Molecular Cloning

Functional Genomics: RNAi, CRISPR, lentiviral packaging and transduction

Microbiology: Experience with BSL-1,2 organisms, Bacterial & Insect Cell Culture, Transformation

Computer skills: GraphPad Prism (statistical), FlowJo (flow cytometry), Adobe Photoshop, MS Office

Instrument expertise: Roche LightCycler® 96, Promega Glomax® Luminometer, Beckman Coulter FC500, BD LSR Fortessa, ViiA7 Real Time PCR system, Olympus FV10i Confocal microscope

Biography of SARAVANA KUMAR KAILASAM MANI JCRI